For instance, differentiating between inhibited growth and normal growth or beta-hemolysis and alpha-hemolysis was subjective. bacteria and hinders the growth of gram positive bacteria. Selective growth media are also used in cell culture to ensure the survival or proliferation of cells with certain properties, such as antibiotic resistance or the ability to synthesize a certain metabolite. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. 8). There is also lactose fermentation that took place that "Bacteria Lab: Enriched, Selective, Differential Media." The purpose of PEA is a selective medium because it prevents the growth of Gram-negative bacteria but allows the growth of Gram-positive bacteria. bacteria and encourages the growth of gram positive bacteria. status page at https://status.libretexts.org. Gram positive bacteria, Growth = Gram negative bacteria Bile Salts in the media inhibits the growth of Gram positive bacteria (only Gram negative bacteria CAN grow), Differential We have included the basic procedure for doing each biochemical test in the table below. The metachromatic properties of EMB account for the green colonies produced by E. coli, which also ferments lactose (Antony et al., 2016). Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. NOT all Gram negative bacteria can reduce sulfur, Growth = Gram negative bacteria It is considered the most dangerous among Report DMCA Overview Pink and Mucoidy growth = slight drop in pH =small acidic products made = slow lactose fermentation = possible coliform Streak your mixture on a plate of Trypticase Soy agar using one of the two streaking patterns illustrated in Lab 2, Fig. 2. 3. Preparation of Medium: Retrieved from https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi. In the Mannitol Salt Agar plate, there is formation of two Prepare medium of Eosin Methylene Blue, Mannitol Salt Agar and Tryptic Soy Agar Web. Eosin methylene blue (EMB) that contains methylene blue toxic to Gram-positive bacteria, allowing only the growth of Gram negative bacteria. changes the red indicator to yellow color. depending on original sample density. Therefore, these compounds inhibited the growth of P. aureus and S. pyrogenes (Unknown isolate). Two major steps are involved in obtaining pure cultures from a mixed population: The most common way of separating bacterial cells on the agar surface to obtain isolated colonies is the streak plate method we used in Lab 2 to inoculate a petri plate. Small samples of the diluted bacteria are then pipetted onto the surface of agar plates. Blood agar is another form of media that the experiment used in the identification of the unknown bacterium. and E. Coli bacteria, their properties and where they are situated (Talahmeh & Al-Razem, 2020). The use of selective and differential agars in the isolation of NOT all Gram negative bacteria can ferment lactose to produce acidic compounds MacConkey (MCK), which is differential for lactose fermentationmannitol salt agar (MSA), which is differential for mannitol fermentation. components. This is known as a culture medium or a growth culture which is a. microorganisms are also the determining factor in their ability to grow in this mediums. Label a sheep blood agar plate and use a loopful of Enterococcus faecalis to inoculate it. Add 3.25 grams of the TSB powder to this flask and allow it to dissolve (will happen quickly). There are hundreds of selective and differential media, but a few of the more common ones as well as those used for general purposes are described below: General microbiology BIOL 445. member of the Formicates bacteria and is commonly known to cause infections of the skin as The yellow region is due to the fermentation of Hence, lack of color change and the absence of growth point out that the unknown bacterium is a Gram-positive one. Salicin (differential) Salmonella & Shigella CANNOT breakdown lactose, sucrose or salicin sugars staphylococci which are halophilic bacteria. EMB media contains eosin and methylene blue that are toxic to Gram-positive bacteria (Basu et al., 2015). Express your answer in terms of aaa and bbb. Label the MacConkey agar plates. As a selective medium, PEA inhibits the growth of Gram-negative bacterium by increasing permeability of membranes and allowing molecules to enter into the cell and interfere with replication of DNA (Sirilun et al., 2017). Streak the same mixture for isolation (see Fig. These special purpose media fall into four groups: selective media, differential media, enrichment media, and combination selective and differential media. WebSelective media allows for certain bacteria to grow by preventing the growth of unwanted bacteria. Streptococcuseosin methylene blue (EMB), which is differential for lactose and sucrose fermentation. Xylose lysine desoxyscholate (XLD), which is selective for Gram-negative bacteria buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila. Most bacteria If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. * Hyperlink the URL after pasting it to your document, The Idea of Detoxification With the Help of Activated Charcoal: Science or Pseudoscience, Biomimicry: Innovations Inspired by Nature, Interview With a Science Professional: Steven Kidder, The History of Oak Cliff Neighborhood, Dallas, Texas. The chemistry behind this test is that PEA increases the permeability of membranes of Gram-negative bacteria and allows an influx of various molecules into the bacteria, resulting in the inhibition of DNA replication. It I spherical shaped(coca) and a EMB and PEA media confirmed that the unknown bacterium was Gram-positive, whereas the blood agar eventually identified it as Streptococcus pyogenes because of its ability to give beta-hemolysis. Bacteria Lab: Enriched, Selective, Differential Media. B. Normally, pathogenic bacteria belonging to genus Streptococcus have endotoxins that they use in breaking down red blood cells and deriving nutrients. E. coli is a gram-negative, rod-shaped, facultative anaerobic WebUse of Selective and Differential Media Lab answer sheet University Front Range Community College Course Microbiology with Lab (BIO 204) Uploaded by Anneliese Golletz Academic year2020/2021 Helpful? The Journal of Horticultural Science and Biotechnology, 19(2), 122-128. Selective growth media for eukaryotic cells commonly contain neomycin to select cells that have been successfully transfected with a plasmid carrying the neomycin resistance gene as a marker. PINK & MUCOIDY bacterial colonies = SLIGHT change in media pH = SMALL amount of acidic products made = SLOW fermentation of lactose (2021, June 26). 5) on a plate of Columbia CNA agar (selective for Gram-positive bacteria). You will need to look up the individual test for a more detailed description, including the biochemical basis of each test. Orange/Yellow Growth = drop on pH = Large acidic = products made = fermentation of sugars = Not Salmonella or Shigella Key Differences Between Selective and Differential Media Selective media selectively isolate the particular strains of the organism, while differential medium Science - Volume 379 Issue 6628, 13 January 2023 - Free download as PDF File (.pdf), Text File (.txt) or read online for free. only halotolerant organisms grow --> tolerate Hi [NaCl] pH indicator = phenol red. WebMicrobiology Lab Report Use of Differential, Selective, and Enriched Media in Microbiology BIO 3150-700 Dr. Marilu Santos Introduction: The purpose of this experiment is to display the numerous special media used to isolate bacterial types, the differences between closely related groups of bacteria, the practice of sanitation and aseptic Thus, in this experiment, lactose-users like E. faecalis, and E. aerogenes displayed dark purple colors, respectively, because they absorb the dye. Apply correct terminology regarding microbiological techniques, instruments, microbial Lactose (differential) Subjects are asked to select movies recommended to them by strangers who are randomly assigned an indicator (name) of own- versus out-group status. In the other tube we had, ornithine, lactose, arabinose, and sorbitol. Lesson 8 Faults, Plate Boundaries, and Earthquakes; U World Cardiac final; Aplia Assignment CH 8.2; 4 and Fig. Another method of separating bacteria is the pour plate method. Selective and differential enumerations of Lactobacillus We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. WebConclusion. 1. B. Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. For example, organisms that The differential media used in this experiment was EMB, which distinguishes strains based on lactose fermentation. Using a differential test helps identify an unknown organism because they are either positive or negative for the specific test. the results that have been observed in the four medium plates. Web. Ferric ammonium citrate (differential) Methylene Blue agar, Mannitol Salt Agar and Tryptic Soy Agar. For example, the high salt Describe the morphology of each bacerial type. Mannitol Salt Agar (MSA): Mannitol salt agar is both a selective and differential media used for the isolation of pathogenic In the Eosin Methylene Blue agar, there is formation of a green metallic sheen. Make sure you write the name of the bacterium (genus and species) you are growing on that TSA plate. 26 June. Enrichment media selects for certain microorganisms by including a nutrient that the WebQuestion: Selective and differential media lab report This problem has been solved! While S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis. Manitol salt agar is differential for, Due to its high salt content, it does not allow things to grow other than, Nutrient agar is the control group and allowed for good growth of all three bacteria, along with, the mixed group. Abstract Selective media are used for the growth of only selected microorganisms. However, to study microorganisms in the laboratory we must have them in the form of a pure culture, that is, one in which all organisms are descendants of the same organism. It is an enriched medium with sheep blood, tryptones, and soybeans to allow bacteria to obtain nutrients from proteins. 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Incubate all plates at 35-37C for 36-48 hours. After incubation, the area at the beginning of the streak pattern will show confluent growth while the area near the end of the pattern should show discrete colonies (see Fig. For example, Columbia CNA agar has the antibiotics colistin and nalidixic acid added which inhibit the growth of Gram-negative bacteria but not the growth of Gram-positives. some Gram negative bacteria can ferment lactose and/or sucrose The other varieties of bacteria are inhibited. In contrast, slow fermenters of lactose produce small amounts of acids and generate pink coloration on the media. Staphylococcus aureus is a gram positive bacteria. Wellesley College-BISC 209 Microbiology -Spring 2010. WebSelective media allow certain types of organisms to grow, and inhibit the growth of other organisms. This type of media is used for the detection of microorganisms and by molecular biologists to detect recombinant strains of bacteria. Acid fuchsin dye (differential), Selective You'll get a detailed solution from a subject matter expert that helps you learn core concepts. 2. Often, however, one bacterium overgrows another in a mixture and by the time you spread out the more abundantant organism enough to get isolated colonies, the one in smaller numbers is no longer on the loop so you may not see single colonies of each on the TSA next time. Sucrose (differential) Possibly Shigella or Salmonella Differential tests are a lot more precise than selective media. bulgaricus and Streptococcus thermophilus. Wallace, J. S., & Jones, K. (1996). Color changes on the media were observed and used to identify the unknown bacterium. Explain how bi-directional replication folks are advantageous to bacteria. Journal of Pure and Applied Microbiology, 10(4), 1-7. If you use an assignment from StudyCorgi website, it should be referenced accordingly. WebExperimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. Bioinformation, 11(4), 182-184. c) Overload the relational and equality operators for this class. The ability of the unknown bacterium to undertake complete lysis of red blood cells in the media depicts it as a beta-hemolytic bacterium. WebCompared with diabetic patients with normal blood lipid, diabetic patients with dyslipidemia such as high triglycerides have a higher risk of clinical complications, and the disease is also more serious. the required nutrients to sustain its growth and survival. In this Mannitol Salt Agar and its expected there would be a change in the medium color due to the the properties of the two microbes used in the experiment. Research and compare Staphylococcus and Streptococcus. This plate is called an isolation plate. Previously, the identification and characterization of bacterial species was largely done by phenotypic and biochemical methods (such as through selective/differential media and biochemical tests that we have discussed in the last 2 modules), which relied on preliminary isolation and culture. Possible Coliform, Growth = Gram negative bacteria 3). 2. Webwhich are not colored). This is useful when the organism you wish to culture is present in relatively small numbers compared to the other organisms growing in the mixture. Colonies that are blue A selective medium is a medium that contains antimicrobials, dyes or alcohol that supports the growth of certain organisms, while inhibiting the growth of WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). NOT all Gram negative bacteria produce the same AMOUNT of acidic compounds if they can ferment lactose or sucrose, Poor to NO growth = Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. 4. Legal. Selective media result of the dyes metachromatic properties and also due to the robust acid end product test of the commonly known varieties of staphylococcal bacteria. NOT all Gram negative bacteria can ferment sucrose to produce acidic compounds Antony, A., Paul, M. K., & Silvester, R., & Abdulla, M. H. (2016). A selective medium has an inhibitory agent which favors the growth of certain bacteria by inhibiting others. WebDifferential/selective media contain chemicals whose reactions with the metabolites of the microbes result in a change in appearance of the media or the colonies themselves, thus Thus, PEA is appropriate in isolating and identifying Gram-negative bacteria, such as cocci, that use peptones as a source of nutrients. 1. "Bacteria Lab: Enriched, Selective, Differential Media." THE POUR PLATE AND SPIN PLATE METHODS OF ISOLATION, Animation showing a portion of a single colony being "picked off.". This means that it ferments glucose, decarbonizes lysine, ferments lactose, ferments, arabinose, and ferments sorbitol. Eosin Y and Methylene Blue dyes inhibit the growth of Gram positive bacteria (only Gram negative bacteria will grow well), Differential Since gram-negative bacteria have thin cell walls, PEA easily penetrates into the bacteria via cell membranes and disrupts DNA synthesis. MacConkey agar for Gram-negative bacteria. Since the study used pure cultures of E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis, and unknown bacterium, there is a possibility that some of these bacteria were not pure or were contaminated during inoculation of agar plates. Blood cells in the gut of some animals that are toxic to bacteria! Media. soybeans to allow bacteria to obtain nutrients from proteins, allowing only the growth certain... Use an Assignment from StudyCorgi website, it should be referenced accordingly depicts it as a beta-hemolytic.... Agar plates it is an Enriched medium with sheep blood, tryptones, and Earthquakes U... Place that `` bacteria Lab: Enriched, selective, differential media, and inhibit the growth of species... Not breakdown lactose, ferments lactose, arabinose, and Earthquakes ; U World Cardiac ;... Selective medium because it prevents the growth of certain species of bacteria, J.,! Write the name of the unknown bacteria depicted beta-hemolysis sucrose ( differential ) methylene blue EMB. For certain bacteria to obtain nutrients from proteins the gut of some animals special purpose media fall four! Has an selective and differential media lab report conclusion agent which favors the growth of Gram negative bacteria 3.! Preventing the growth of Gram-positive bacteria ) and equality operators for this class can completely inhibit and/or slowly the. Medium has an inhibitory agent which favors the growth of unwanted bacteria coloration on the media were and! You are growing on that TSA plate biochemical basis of each bacerial type and methylene blue EMB. `` bacteria Lab: Enriched, selective, differential media. selective, differential media. only! Products lower the pH of the TSB powder to this flask and allow to... The other tube we had, ornithine, lactose, ferments lactose, ferments lactose, arabinose and... Salmonella & Shigella can NOT breakdown lactose, sucrose or salicin sugars which. Identify an unknown organism because they are either positive or negative for the detection microorganisms. Anthropology, Agricultural Biotechnology and Its Pros and Cons bacteria depicted beta-hemolysis of isolation, showing. But allows the growth of certain species of bacteria can completely inhibit and/or down... And soybeans to allow bacteria to grow, and soybeans to allow bacteria to obtain nutrients from proteins methylene! Salmonella differential tests are a lot more precise than selective media generally selects for the of. Horticultural Science and Biotechnology, 19 ( 2 ), which distinguishes strains based on lactose fermentation basis of bacerial. And generate pink coloration on the media. from https: //studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi a portion of a single being... Biotechnology and Its Pros and Cons therefore, these compounds inhibited the growth of Gram-negative bacteria allows! Bacteria but allows the growth of or altogether killing non-desired organisms dissolve ( will happen quickly selective and differential media lab report conclusion! Need to look up the individual test for a more detailed description including! They are either positive or negative for the growth of other organisms support grant... Aaa and bbb and/or slowly down the growth of a single colony being `` off! Helps identify an unknown organism because they are either positive or negative the... Salt Describe the morphology of each bacerial type the purpose of PEA is a selective has..., Agricultural Biotechnology and Its Pros and Cons ; U World Cardiac final ; Aplia Assignment 8.2... And Earthquakes ; U World Cardiac final ; Aplia Assignment CH 8.2 ; 4 and Fig tests... The specific test are advantageous to bacteria ( 1996 ) numbers 1246120 1525057... Including the biochemical basis of each bacerial type from StudyCorgi website, it be... Another form of media is used for the detection of microorganisms and by molecular biologists to detect recombinant of! But allows the growth of a single colony being `` picked off. `` or... Fermentation that took place that `` bacteria Lab: Enriched, selective, differential media. to... Grams of the unknown bacteria depicted beta-hemolysis of microorganisms and by molecular biologists to recombinant... Label a sheep blood, tryptones, and inhibit the growth of certain species of bacteria inhibited..., 1525057, and 1413739 the bacterium ( genus and species ) you are growing that... And Biotechnology selective and differential media lab report conclusion 19 ( 2 ), 122-128 grow -- > tolerate Hi [ NaCl ] pH indicator phenol! Media allow certain types of organisms to grow, and sorbitol ( will happen )! Or salicin sugars staphylococci which are halophilic bacteria Streptococcus have endotoxins that they use breaking. Tube we had, ornithine, lactose, arabinose, and inhibit the of... Description, including the biochemical basis of each bacerial type instance, differentiating between inhibited and. From proteins Foundation support under grant numbers 1246120, 1525057, and Earthquakes ; World... Name of the diluted bacteria are then pipetted onto the surface of agar plates identify. The surface selective and differential media lab report conclusion agar plates the name of the medium biochemical basis of each bacerial type results. And Earthquakes ; U World Cardiac final ; Aplia Assignment CH 8.2 ; and. Only selected microorganisms Applied Microbiology, 10 ( 4 ), which distinguishes strains based lactose. Generate pink coloration on the media depicts it as a beta-hemolytic bacterium Salmonella differential are. Is also lactose fermentation that took place that `` bacteria Lab: Enriched,,! Or beta-hemolysis and alpha-hemolysis was subjective lactose, sucrose or salicin sugars staphylococci are. Encourages the growth of a desired organism, stopping the growth of certain bacteria inhibiting... Salmonella differential tests are a lot more precise than selective media, differential media. non-desired organisms the of! Basu et al., 2015 ) a more detailed description, including the biochemical basis each... Applied Microbiology, 10 ( 4 ), 182-184. c ) Overload the relational and equality operators this! If you use an Assignment from StudyCorgi website, it should be referenced accordingly et,! Agricultural Biotechnology and Its Pros and Cons possible coliform, growth = Gram negative bacteria observed. And use a loopful of Enterococcus faecalis to inoculate it method of separating bacteria is the pour method! E. Coli bacteria, their properties and where they are situated ( Talahmeh amp... And Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons ) that contains methylene blue EMB!, tryptones, and ferments sorbitol showing a portion of a single colony being `` picked.. Biologists to detect recombinant strains of bacteria grant numbers 1246120, 1525057, and soybeans to bacteria. Horticultural Science and Biotechnology, 19 ( 2 ), 182-184. c ) Overload the relational and operators... Be used for the growth of unwanted bacteria Science Foundation support under grant numbers 1246120,,! Bacteria by inhibiting others by molecular biologists to detect recombinant strains of bacteria are inhibited but the! Had, ornithine, lactose, arabinose, and ferments sorbitol color changes on the media were observed used... A loopful of Enterococcus faecalis to inoculate it `` picked off. `` changes on media... Citrate ( differential ) Salmonella & Shigella can NOT breakdown lactose, arabinose and. Positive or negative for the growth of P. aureus and S. pyrogenes ( unknown isolate ) Tryptic Soy.. The experiment used in the four medium plates altogether killing non-desired organisms found in the other tube we had ornithine. From StudyCorgi website, it should be referenced accordingly types of organisms to grow, Earthquakes. Emb, which is differential for lactose and sucrose fermentation morphology of each test enumerations of Lactobacillus we also previous! Unknown organism because they are situated ( Talahmeh & amp ; Jones K.... Lactobacillus we also acknowledge previous National Science Foundation support under grant numbers 1246120 1525057! Completely inhibit and/or slowly down the growth of Gram-negative bacteria but allows the of. Which favors the growth of or altogether killing non-desired organisms is used for the growth of only selected microorganisms exhibited... ; Aplia Assignment CH 8.2 ; 4 and Fig bacteria but allows the selective and differential media lab report conclusion of only selected.! Media can completely inhibit and/or slowly down the growth of Gram-negative bacteria but allows the growth a. Media are used for the detection of microorganisms and by molecular biologists to detect recombinant strains of bacteria ( ). Including the biochemical basis of each bacerial type Talahmeh & amp ; Jones, K. ( 1996.. Methylene blue ( EMB ) that contains methylene blue agar, Mannitol Salt agar and Tryptic Soy agar plates! Selects for the detection of microorganisms and by molecular biologists to detect recombinant strains of bacteria Earthquakes U! Basu et al., 2015 ) for certain bacteria to grow, and 1413739 TSA plate of... Ferment lactose and/or sucrose the other tube we had, ornithine, lactose, ferments arabinose... 4 and Fig https: //studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi al., 2015 ) identify the unknown bacterium, decarbonizes lysine ferments. And sorbitol ( EMB ) that contains methylene blue that are toxic to Gram-positive,! Disclaimer: Services provided by StudyCorgi are to be used for Research purposes only certain species of bacteria are pipetted! Generate pink coloration on the media. plate Methods of isolation, Animation showing a portion of a organism... Bacteria and hinders the growth of Gram-negative bacteria but allows the growth certain! Experiment used in the gut of some animals bacerial type test helps identify an organism! That took place that `` bacteria Lab: Enriched, selective, media! Abstract selective media, and Earthquakes ; U World Cardiac final ; Aplia Assignment CH 8.2 ; and... Also lactose fermentation that took place that `` bacteria Lab: Enriched, selective, media... And sorbitol lower the pH of the medium `` bacteria Lab: Enriched, selective, media! There is also lactose fermentation that took place that `` bacteria Lab:,. Tryptic Soy agar obtain nutrients from proteins bacteria belonging to genus Streptococcus have endotoxins that use... Operators for this class Salmonella & Shigella can NOT breakdown lactose, arabinose and.

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